Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing

被引：206

作者：

Azevedo, Ana M. ^{[1
]}

Rosa, Paula A. J. ^{[1
]}

Ferreira, I. Filipa ^{[1
]}

Aires-Barros, M. Raquel ^{[1
]}

机构：

[1] Univ Tecn Lisboa, Ctr Biol & Chem Engn, Inst Biotechnol & Bioengn, Inst Super Tecn, P-1049001 Lisbon, Portugal

来源：

TRENDS IN BIOTECHNOLOGY | 2009年 / 27卷 / 04期

关键词：

MONOCLONAL-ANTIBODIES; PLASMID DNA; LARGE-SCALE; PURIFICATION; SYSTEMS; DOWNSTREAM; EXTRACTION; SEPARATION; HYBRIDOMA; PROTEINS;

D O I：

10.1016/j.tibtech.2009.01.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

The therapeutic use of proteins has created an increasing demand for feasible and economical methods for both up- and downstream processes. However, whereas upstream processes have attracted substantial investment and commercial attention, downstream processing has been overlooked, causing a production bottleneck that is shifting the costs of production. This review focuses on the use of aqueous two-phase extraction as an option for the downstream processing of therapeutic proteins. It is a potential and promising liquid-liquid extraction technique for the purification of biomolecules, such as monoclonal antibodies, growth factors and hormones, that combines a high selectivity and biocompatibility with an easy scale-up and continuous operation mode.

引用

页码：240 / 247

页数：8

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