Probing biocatalytic transformations with CdSe-ZnS QDs

被引：167

作者：

Gill, Ron

Freeman, Ronit

Xu, Jian-Ping

Willner, Itamar ^{[1
]}

Winograd, Shira

Shweky, Itzik

Banin, Uri

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Ctr Nanosci & Nanotechnol, IL-91904 Jerusalem, Israel

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 48期

关键词：

D O I：

10.1021/ja066636t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

CdSe/ZnS QDs enable the optical probing of the biocatalytic oxidation of tyrosine derivatives and of the scission of peptides by thrombin. CdSe/ZnS QDs were modified with tyrosine methyl ester or with a tyrosine-containing peptide. The tyrosine units were reacted with tyrosinase/O2 to yield the respective l-DOPA and quinone derivatives. The luminescence of QDs modified by the enzyme-generated quinone units is quenched. The quinone-functionalized peptide associated with the QDs was cleaved by thrombin, a process that restored the luminescence of the QDs. Copyright © 2006 American Chemical Society.

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页码：15376 / 15377

页数：2

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