Determination of cell number and size of a population of Pseudomonas fluorescens by image analysis

被引：8

作者：

Azeredo, J ^{[1
]}

Meinders, J ^{[1
]}

Feijo, J ^{[1
]}

Oliveira, R ^{[1
]}

机构：

[1] UNIV MINHO,CTR ENGN BIOL,P-4709 BRAGA,PORTUGAL

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 05期

关键词：

D O I：

10.1023/A:1018439916772

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microscopic observations of a population of Pseudomonas fluorescens were digitised by a frame grabber and an appropriate threshold was chosen to extract the objects from the background. Fully grown, single bacteria were normally distributed around two mean sizes. Two Gauss functions were fitted by least square analysis to these points and the enumeration of single cells was obtained from the area of each Gauss curve and made for each threshold selected. The number of particles counted was constant over a large range of threshold (80-180) whereas the cell area increased with the threshold installed.

引用

页码：355 / 358

页数：4

共 8 条

[1] INSITU ENUMERATION OF BACTERIAL ADHESION IN A PARALLEL PLATE FLOW CHAMBER - ELIMINATION OR IN FOCUS FLOWING BACTERIA FROM THE ANALYSIS [J].