Identification of the glycosyl transferase required for synthesis of the principal glycolipid characteristic of heterocysts of Anabaena sp strain PCC 7120

被引:29
作者
Awai, Koichiro
Wolk, C. Peter [1 ]
机构
[1] Michigan State Univ, US DOE, Plant Res Lab, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA
[3] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA
关键词
heterocyst; glycosyl transferase; heterocyst envelope glycolipid;
D O I
10.1111/j.1574-6968.2006.00512.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nitrogenase is oxygen-labile. Cyanobacterial heterocysts can fix N-2 in an oxic milieu because their interior is micro-oxic, for which the glycolipid layer of the heterocyst envelope is required. ORF all5341 of the Anabaena sp. genome predicts a glycosyl transferase. An insertional mutant of all5341 synthesized only a nonglycosylated form of heterocyst envelope glycolipid, and lacked a glycolipid layer. All5341 appears to be the transferase required to glycosylate the glycolipid aglycone.
引用
收藏
页码:98 / 102
页数:5
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