Quadruple immunofluorescence: A direct visualization method

被引：20

作者：

Ferri, GL

Gaudio, RM

Castello, IF

Berger, P

Giro, G

机构：

[1] OASI IRCCS,TROINA,ITALY

[2] AUSTRIAN ACAD SCI,INST BIOMED AGING RES,INNSBRUCK,AUSTRIA

[3] CNR,IST FRAE,I-40126 BOLOGNA,ITALY

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1997年 / 45卷 / 02期

关键词：

immunohistochemistry; quadruple immunostaining; multiple immunostaining; fluorochromes; interference filters; co-localization;

D O I：

10.1177/002215549704500201

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We describe fluorescence immunostaining of four different antigens in the same section. The fluorochrome conjugates used show highest emission in the blue, green, yellow, and red regions of the visible light spectrum, respectively. Specially designed single fluorochrome filter combinations allow selective visualization of each fluorochrome label in turn, without visible crosstalk with the others.

引用

页码：155 / 158

页数：4

共 12 条

[1]

BERGER P, 1988, INFLUENCE NEW TECHNO, P212

[2]

BRELJE TC, 1993, METHOD CELL BIOL, V38, P97

[3] THE VGF PROTEIN IN RAT ADENOHYPOPHYSIS - SEX-DIFFERENCES AND CHANGES DURING THE ESTROUS-CYCLE AND AFTER GONADECTOMY [J].