Micellar electrokinetic capillary chromatography for the separation of phenoxymethylpenicillin and related substances

被引：15

作者：

Zhu, YX ^{[1
]}

VanSchepdael, A ^{[1
]}

Roets, E ^{[1
]}

Hoogmartens, J ^{[1
]}

机构：

[1] KATHOLIEKE UNIV LEUVEN,FAC FARMACEUT WETENSCHAPPEN,LAB FARMACEUT CHEM ANAL GENEESMIDDELEN,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 781卷 / 1-2期

关键词：

buffer composition; pharmaceutical analysis; phenoxymethylpenicillin; penicillins; antibiotics;

D O I：

10.1016/S0021-9673(97)00311-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A micellar electrokinetic capillary chromatography (MECC) method has been developed for the separation of phenoxymethylpenicillin and its related substances. This method permits phenoxymethylpenicillin to be completely separated from eight of its related substances. The separation buffer contained 40 mM sodium dihydrogen phosphate and 100 mM sodium dodecyl sulfate (SDS) adjusted to pH 7.0. The influence of various parameters on the separation such as pH of the buffer, SDS concentration, buffer concentration, temperature, voltage and different length and internal diameter of capillary were investigated. The method shows good repeatability, linearity and sensitivity. It is suitable not only for the related substances test but also for the assay. The results obtained by MECC were also compared with those obtained by liquid chromatography. The analysis of variance showed no significant difference between the results obtained by the two methods. (C) 1997 Elsevier Science B.V.

引用

页码：417 / 422

页数：6

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