Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates from nonanoic acid by Pseudomonas putida KT2440

被引：103

作者：

Sun, Zhiyong

Ramsay, Juliana A.

Guay, Martin

Ramsay, Bruce A. ^{[1
]}

机构：

[1] Polyferm Canada Inc, RR1, Harrowsmith, ON K0H 1V0, Canada

[2] Queens Univ, Dept Chem Engn, Kingston, ON K7L 3N6, Canada

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 74卷 / 01期

关键词：

Pseudomonas putida; fed-batch; high-density; fermentation; PHA;

D O I：

10.1007/s00253-006-0655-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Pseudomonas putida KT2440 grew on glucose at a specific rate of 0.48 h(-1) but accumulated almost no poly3- hydroxyalkanoates (PHA). Subsequent nitrogen limitation on nonanoic acid resulted in the accumulation of only 27% medium-chain-length PHA (MCL-PHA). In contrast, exponential nonanoic acid-limited growth (mu = 0.15 h(-1)) produced 70 g l(-1) biomass containing 75% PHA. At a higher exponential feed rate (mu = 0.25 h(-1)), the overall productivity was increased but less biomass (56 g l(-1)) was produced due to higher oxygen demand, and the biomass contained less PHA (67%). It was concluded that carbon-limited exponential feeding of nonanoic acid or related substrates to cultures of P. putida KT2440 is a simple and highly effective method of producing MCL-PHA. Nitrogen limitation is unnecessary.

引用

页码：69 / 77

页数：9

共 38 条

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