13C CPMAS studies of plant cell wall materials and model systems using proton relaxation-induced spectral editing techniques

The solid state C-13 CPMAS NMR spectra of plant cell walls are often complex owing to superposition of resonances from different polysaccharides and the heterogeneity of the cell wall assembly. In this paper, rye describe the application of a set of proton relaxation-induced spectral editing (PRISE) experiments which combine H-1 relaxation properties (T-1, T-1 rho, T-2) with high resolution spectroscopy (CPMAS) to relate the dynamics of the plant cell walls and model systems to their domain structural derails. with PRISE it has buen found that in plant cell wall materials, cellulose is always associated with the long components of spin-lattice relaxation in both the laboratory and rotating frames whereas non-cellulose polysaccha- rides (pectin and hemicellulose) are associated with the short ones. For the proton T-2 relaxation, cellulose is only associated with the short component (below 20 mu s), pectin contributes to both the short component and the long one. (C) 2000 Elsevier Science B.V. All rights reserved.