A new cholesterol derivative suitable for transfecting certain type of cells in the presence of 10% serum

被引：21

作者：

Sochanik, A

Kaida, I

Mitrus, I

Rajca, A

Szala, S

机构：

[1] Marie Sklodowska Curie Mem Inst, Ctr Oncol, Dept Mol Biol, PL-44100 Gliwice, Poland

[2] Natl Acad Sci Ukraine, Inst Mol Biol & Genet, Dept Mol Oncogenet, Kiev, Ukraine

[3] Silesian Tech Univ, Inst Organ Chem & Technol, Gliwice, Poland

来源：

CANCER GENE THERAPY | 2000年 / 7卷 / 04期

关键词：

cationic cholesterol; gene transfection; 10% serum;

D O I：

10.1038/sj.cgt.7700167

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a new cationic lipid suitable for use as a DNA carrier in the presence of 10% sera. The novel compound (abbreviated as Arg-Chol) contains cholesterol and a dipeptide consisting of glycine and sterically protected arginine. The efficiency of reporter gene transfection using liposomes based on this new reagent was compared with that of liposomes made with other cationic derivatives of cholesterol. Lipoplexes formulated with the newly synthesized lipid mediate in vitro transfection of B16(F10) murine melanoma cells in the presence of 10% sera more efficiently than in other cell lines and compared with other cholesterol derivatives studied.

引用

页码：513 / 520

页数：8

共 22 条

[1] The effects of cholesterol inclusion on the vesicular membranes of cationic lipids [J].