IMPORTANCE OF THE LIPID PEROXIDATION BIOMARKERS AND METHODOLOGICAL ASPECTS FOR MALONDIALDEHYDE QUANTIFICATION

被引：336

作者：

Grotto, Denise ^{[1
]}

Maria, Lucas Santa ^{[1
]}

Valentini, Juliana ^{[1
]}

Paniz, Clovis ^{[1
]}

Schmitt, Gabriela ^{[1
]}

Garcia, Solange Cristina ^{[1
]}

Pomblum, Valdeci Juarez ^{[2
]}

Rocha, Joao Batista T. ^{[3
]}

Farina, Marcelo ^{[4
]}

机构：

[1] Univ Fed Santa Maria, Dept Anal Cin & Toxicol, BR-97110970 Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Clin Med, BR-97110970 Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Dept Bioquim Toxicol, BR-97110970 Santa Maria, RS, Brazil

[4] Univ Fed Santa Catarina, Dept Bioquim, BR-88040900 Florianopolis, SC, Brazil

来源：

QUIMICA NOVA | 2009年 / 32卷 / 01期

关键词：

oxidative stress; MDA; methodologies; PERFORMANCE LIQUID-CHROMATOGRAPHY; THIOBARBITURIC ACID REACTION; TOTAL PLASMA MALONDIALDEHYDE; POLYUNSATURATED FATTY-ACIDS; OXIDATIVE STRESS; GAS-CHROMATOGRAPHY; RAT-LIVER; BOUND MALONDIALDEHYDE; MALONALDEHYDE; ALDEHYDES;

D O I：

10.1590/S0100-40422009000100032

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.

引用

页码：169 / 174

页数：6

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