Modulation of noradrenergic and serotonergic transmission by noxious stimuli and intrathecal morphine differs in the dorsal raphe nucleus of anesthetized rat: in vivo voltammetric studies

We examined the effects of cutaneous noxious heat as well as the intrathecal administration of morphine on the oxidation current of peaks 1 and 2 in the dorsal raphe nucleus (DRN) of anesthetized rats. Differential normal pulse voltammetry with carbon fiber electrodes identified distinct oxidation currents at +120 mV (peak 1: catechol signals) and +280 mV (peak 2: 5-hydroxyindole signals). The catechol signal was significantly increased by 22.9+/-4.2% after applying cutaneous noxious heat at 52 degreesC. The 5-hydroxyindole signal was decreased by 39.8+/-4.3 and by 25.2+/-4.7% after stimulation with cutaneous noxious heat at 52 and 45 degreesC, respectively. A low dose of morphine (2.5 mug) potentiated the increase in the catechol signal and the decrease in the 5-hydroxyindole signal induced by noxious heat, and a high dose (10.0 mug) attenuated both. The effects of morphine at low (2.5 mug) and high doses (10.0 mug) were antagonized by naloxone (0.5 mg/kg, i.p.). These results indicate that noxious heat stimulation increased the catechol signal and decreased the 5-hydroxyindole signal in the DRN. The intrathecal administration of morphine affects the noxious stimulation-induced activity of noradrenergic and serotonergic neurotransmission in the DRN. (C) 2002 Elsevier Science Ireland Ltd. and the Japan Neuroscience Society. All rights reserved.