Variation of cytokine patterns related to therapeutic response in diffuse cutaneous leishmaniasis

被引:46
作者
Bomfim, G
Nascimento, C
Costa, J
Carvalho, EM
BarralNetto, M
Barral, A
机构
[1] UNIV FED BAHIA,FAMED,HUPES,SERV IMUNOL,BR-41170290 SALVADOR,BA,BRAZIL
[2] FIOCRUZ MS,CTR PESQUISAS GONCALO MONIZ,BR-40295001 SALVADOR,BA,BRAZIL
[3] UNIV FED MARANHAO,FAC MED,DEPT DOENCA INFECCIOSAS & PARASITARIAS,BR-65020 SAO LUIS,MARANHAO,BRAZIL
关键词
leishmaniasis; cytokines; therapy; cutaneous leishmaniasis;
D O I
10.1006/expr.1996.0104
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Diffuse cutaneous leishmaniasis is a rare entity characterized by disseminated cutaneous nodules associated with specific anergy to leishmanial antigens. A low but not absent IFN-gamma expression by cells present in cutaneous lesions has been documented during the active phase of diffuse cutaneous leishmaniasis. In this study we confirm this observation, and extend it by showing a similar pattern in peripheral blood mononuclear cells and the variation of mRNA cytokine expression pattern during different stages of the disease. During active disease, patients did not express mRNA for IFN-gamma, while expressing mRNA for IL-2, IL-4, and IL-10. In contrast, an expression of IFN-gamma and low IL-10 was observed after treatment-induced transient healing of cutaneous lesions. In three patients we have been able to analyze a third PBMC sample obtained after clinical relapse, documenting in all of them decreased IFN-gamma expression with no expression of IL-10. Although there was an association between the appearance of IFN-gamma expression and clinical improvement, with marked expression of IFN-gamma mRNA and decreased expression of mRNA for IL-10 after treatment, this was not sufficient to prevent relapse in these patients. Therefore, it is possible that factors other than the cytokines characteristic of the Th1 and Th2 balance are implicated in the inability of diffuse cutaneous leishmaniasis patients to mount an anti-leishmania immune response causing clinical improvement. (C) 1996 Academic Press, Inc.
引用
收藏
页码:188 / 194
页数:7
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