Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations

被引：2

作者：

Kobayashi, K

Sarrobert, C

Rivero, MM

Maldonado, S

Robaglia, C

Mentaberry, A

机构：

[1] UBA, CONICET, Fac Ciencias Exactas & Nat, Inst Invest Ingn Genet & Biol Mol, RA-1428 Buenos Aires, DF, Argentina

[2] CEA, Lab Metab Carbone, Dept Ecophysiol Vegetale & Microbiol, Ctr Cadarache, F-13108 St Paul Les Durance, France

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2004年 / 42卷 / 09期

关键词：

chloroplast; movement protein; physiology; potexvirus; tobacco;

D O I：

10.1016/j.plaphy.2004.07.010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Transgenic Nicotiana tabacum plants expressing the TGBp1 movement protein of potato virus X (PVX) were studied to investigate the effects caused by this protein on plant physiology and development. TGBp1 caused consistent reductions of size and weight in different organs of these plants; however shoot-to-root ratios were similar to those of control plants. Transgenic seedlings showed smaller root meristems and calli derived from TGBp1 leaves grew at a slower rate through successive subcultures. Microscopic observations of TGBp1 plants revealed flattened chloroplasts containing plastoglobuli-like bodies. Further analyses showed a considerable reduction in photosynthetic rate, lower starch levels in leaves and roots, higher nitrate accumulation in leaves and induction of pathogenesis-related (PR) protein genes. Since these changes were not observed when other PVX sequences were expressed in tobacco, we postulate that TGBp1 is an important symptom contributor in PVX infections. (C) 2004 Elsevier SAS. All rights reserved.

引用

页码：731 / 738

页数：8

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