Analysis of gene expression following spinal cord injury in rat using complementary DNA microarray

被引：66

作者：

Tachibana, T ^{[1
]}

Noguchi, K

Ruda, MA

机构：

[1] NIDCR, Cellular Neurosci Sect, Pain & Neurosensory Mechanisms Branch, NIH, Bethesda, MD 20892 USA

[2] Hyogo Med Univ, Dept Anat & Neurosci, Nishinomiya, Hyogo 6638501, Japan

来源：

NEUROSCIENCE LETTERS | 2002年 / 327卷 / 02期

基金：

美国国家卫生研究院;

关键词：

microarray; spinal cord injury; reverse transcription-polymerase chain reaction; heat shock protein; tissue inhibitor of metalloproteinase; fatty acid-binding protein; lecithin; cholesterol acyltransferase; plasma membrane Ca2+-ATPase;

D O I：

10.1016/S0304-3940(02)00375-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To identify genes that were altered by spinal cord injury (SCI), we used complementary DNA microarray consisting 1176 rat genes. Rats were subjected to contusive injury of the thoracic spinal cord. Sham animals received only a laminectomy. Twenty-four hours later, spinal cord was dissected out, a P-32 labeled probe was prepared and hybridized to the microarray. We identified three genes that showed a greater than 2-fold increase in SCI tissue, heat shock 27-kDa protein, tissue inhibitor of metalloproteinase-1 and epidermal fatty acid-binding protein. Seven genes, lecithin:cholesterol acyltransferase, dipeptidyl aminopeptidase related protein, phospholipase C delta 4, plasma membrane Ca2+ ATPase isoform 2, G-protein G(O) alpha subunit, GABA transporter 3, and neuroendrocrine protein 7132 were downregulated greater than 50% in SCI tissue. Changes in expression of these genes were confirmed by reverse transcription-polymerase chain reaction. These genes may play a role in the response to tissue damage or repair following SCI. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：133 / 137

页数：5

共 23 条

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