Antibacterial steroid alkaloids from the stem bark of Holarrhena pubescens

被引：45

作者：

Chakraborty, A ^{[1
]}

Brantner, AH ^{[1
]}

机构：

[1] Graz Univ, Inst Pharmacognosy, A-8010 Graz, Austria

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 1999年 / 68卷 / 1-3期

关键词：

Holarrhena pubescens; apocynaceae; stem bark; alkaloids; antibacterial activity;

D O I：

10.1016/S0378-8741(99)00119-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Holarrhena pubescens (syn. H. antidysenterica) (L.) WALL. stem bark was tested for antibacterial efficacy against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa using the microdilution broth method as well as the disc diffusion method. The crude methanolic extract was active against all tested bacteria. Further chemical fractionation indicated that the antibacterial activity was mainly associated with the alkaloids. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the crude extract, the total alkaloids and the neutral fraction using microdilution broth method. The results were compared with reference antibiotics. The total alkaloids showed remarkable activity against S. aureus (MIC = 95 mu g/ml). (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：339 / 344

页数：6

共 18 条

[1] IMMUNOMODULATING AGENTS OF PLANT-ORIGIN .1. PRELIMINARY SCREENING [J].