Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotube-modified electrodes

被引:104
作者
Luo, Xiliang [1 ]
Killard, Anthony J. [1 ]
Smyth, Malcolm R. [1 ]
机构
[1] Dublin City Univ, Sch Chem Sci, Natl Ctr Sensor Res, Dublin 9, Ireland
关键词
glucose oxidase; carbon nanotubes; biosensor; direct electrochemistry; reagentless;
D O I
10.1002/elan.200603513
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The direct electrochemistry of glucose oxidase (GOD) was revealed at a carbon nanotube (CNT)-modified glassy carbon electrode, where the enzyme was immobilized with a chitosan film containing gold nanoparticles. The immobilized GOD displays a pair of redox peaks in pH 7.4 phosphate buffer solutions (PBS) with the formal potential of about -455 mV (vs. Ag/AgCl) and shows a surface-controlled electrode process. Bioactivity remains good, along with effective catalysis of the reduction of oxygen. In the presence of dissolved oxygen, the reduction peak current decreased gradually with the addition of glucose, which could be used for reagentless detection of glucose with a linear range from 0.04 to 1.0 mM. The proposed glucose biosensor exhibited high sensitivity, good stability and reproducibility, and was also insensitive to common interferences such as ascorbic and uric acid. The excellent performance of the reagentless biosensor is attributed to the effective enhancement of electron transfer between enzyme and electrode surface by CNTs, and the biocompatible environment that the chitosan film containing gold nanoparticles provides for immobilized GOD.
引用
收藏
页码:1131 / 1134
页数:4
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