Signals from the IL-9 receptor are critical for the early stages of human intrathymic T cell development

被引:30
作者
De Smedt, M
Verhasselt, B
Kerre, T
Vanhecke, D
Naessens, E
Leclercq, G
Renauld, JC
Van Snick, J
Plum, J
机构
[1] Ghent Univ Hosp, Dept Clin Chem Microbiol & Immunol, B-9000 Ghent, Belgium
[2] Ludwig Inst Canc Res, Brussels Branch, Brussels, Belgium
[3] Catholic Univ Louvain, Expt Med Unit, Brussels, Belgium
关键词
D O I
10.4049/jimmunol.164.4.1761
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Highly purified human CD34(+) hemopoietic precursor cells differentiate into mature T cells when seeded in vitro in isolated fetal thymic lobes of SCID mice followed by fetal thymus organ culture (FTOC). Here, this chimeric human-mouse FTOC was used to address the role of IL-9 and of the alpha-chain of the IL-9 receptor (IL-9R alpha) in early human T cell development. We report that addition of the mAb AH9R7, which recognizes and blocks selectively the human high affinity alpha-chain of the IL-9R, results in a profound reduction of the number of human thymocytes, Analysis of lymphoid subpopulations indicates that a highly reduced number of cells undergo maturation from CD34(+) precursor cells toward CD4(+)CD3(-)CD8(-)CD1(+) progenitor cells and subsequently toward CD4(+)CD8(+) double positive (DP) thymocytes. Addition of IL-9 to the FTOC resulted in an increase in cell number, without disturbing the frequencies of the different subsets. These data suggest that IL-9R alpha signaling is critical in early T lymphoid development.
引用
收藏
页码:1761 / 1767
页数:7
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