Assessment of DNA base oxidation and glutathione level in patients with type 2 diabetes

被引:77
作者
Dinçer, Y [1 ]
Akçay, A
Alademir, Z
Ilkova, H
机构
[1] Istanbul Univ, Cerrahpasa Med Fac, Dept Biochem, TR-34300 Istanbul, Turkey
[2] Istanbul Univ, Cerrahpasa Med Fac, Dept Internal Med, Div Endocrinol Diabet & Metab, TR-34300 Istanbul, Turkey
关键词
DNA damage; DNA base oxidation; comet assay; glutathione; diabetes;
D O I
10.1016/S0027-5107(02)00143-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The first aim of the present study was to examine the relationship between reduced glutathione (GSH) level, a powerful cellular antioxidant, and oxidative damage to DNA; and secondly, to see the effect of glycemic control on oxidative DNA damage in type 2 diabetics. We determined GSH level and, using the comet assay, formamidopyrimidine DNA glycosylase (Fpg)-sensitive sites which indicates oxidised guanine in freshly isolated blood from age-matched type 2 diabetics and controls. We found significant differences between men and women in the control group for both GSH and Fpg-sensitive sites. Therefore, we compared the controls and type 2 diabetics separately in men and women. GSH level of whole blood was found to be lower, Fpg-sensitive sites in leukocytes was found to be higher in the both type 2 diabetic men and women, as compared with their respective controls. When the diabetic group was divided into two groups as well-controlled diabetics and poorly-controlled diabetics with respect to glycosylated haemoglobine levels, it was found that Fpg-sensitive sites was significantly higher in the poorly-controlled diabetics than in the well-controlled diabetics in both the men and women. GSH level was lower in the poorly-controlled diabetics but not significantly. Fpg-sensitive sites were found to be moderately correlated with both glycosylated haemoglobine and GSH, and weakly correlated with glucose. Data indicate that decreased GSH level may be a contributory factor for enhanced oxidative DNA damage in type 2 diabetics; and chronic hyperglycemia derived from poorly-controlled diabetic conditions may induce oxidative DNA damage in these patients. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:75 / 81
页数:7
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