cDNA cloning and chromosomal mapping of rat Smad2 and Smad4 and their expression in cultured rat articular chondrocytes

被引：9

作者：

Osaki, M

Tsukazaki, T

Ono, N

Yonekura, A

Hirota, Y

Miyazaki, Y

Shindo, H

Sonta, S

Yamashita, S

机构：

[1] Nagasaki Univ, Sch Med, Atom Bomb Dis Inst, Dept Nat Med, Nagasaki 8528523, Japan

[2] Nagasaki Univ, Sch Med, Dept Orthopaed Surg, Nagasaki, Japan

[3] Aichi Human Serv Ctr, Inst Dev Res, Dept Genet, Aichi, Japan

来源：

ENDOCRINE JOURNAL | 1999年 / 46卷 / 05期

关键词：

Smad2; Smad4; rat; mapping; chondrocyte;

D O I：

10.1507/endocrj.46.695

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Smad proteins are known to transduce signalling of TGF-beta receptor superfamily. We report here the entire sequences of rat Smad2 and Smad4 which have not been identified yet. Entire sequences were identified by degenerated polymerase chain reaction and following phage library screening and 5' RACE. The predicted amino acid sequences of rat Smad2 and Smad4 are highly conserved among rat, human and mouse. We also mapped these Smads to chromosome 18q.12.3. Unlike endothelial cells, TGF-beta 1 stimulates articular chondrocyte proliferation as well as extracellular matrix production, and acts as a repairing agent against cartilage destruction. Since both Smad2 and Smad4 are essential factors for TGF-beta signalling, we examined their expression and regulation in cultured articular chondrocytes. Northern blot analysis showed that TGF-beta 1 significantly increased the mRNA level of Smad2 but not of Smad4 in a dose- and time-dependent manner, suggesting that the augmentation of TGF-beta 1 action is caused by increasing the expression of the downstream signalling molecule.

引用

页码：695 / 701

页数：7

共 23 条

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