PCR assay for the detection and the identification of atypical canine coronavirus in dogs

被引：20

作者：

Pratelli, A ^{[1
]}

Tinelli, A ^{[1
]}

Decaro, N ^{[1
]}

Camero, M ^{[1
]}

Elia, G ^{[1
]}

Gentile, A ^{[1
]}

Buonavoglia, C ^{[1
]}

机构：

[1] Fac Vet Med Bari, Dept Anim Hlth & Wellbeing, I-70010 Bari, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2002年 / 106卷 / 02期

关键词：

dog; coronavirus; PCR; genotypes;

D O I：

10.1016/S0166-0934(02)00165-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. This note describes a PCR assay to identify atypical CCoV strains with nucleotide substitutions in the M gene. A total of 177 faecal samples from dogs CCoV positive previously with the PCR assay were analysed. Sixty-two of the 177 samples were amplified with the PCR described in the present study and were thus considered atypical CCoVs. The specificity of the PCR typing assay was confirmed by sequence analysis of the PCR products. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：209 / 213

页数：5

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