Gap-junctional coupling between neutrophils and endothelial cells: a novel modulator of transendothelial migration

被引:77
作者
Zahler, S [1 ]
Hoffmann, A [1 ]
Gloe, T [1 ]
Pohl, U [1 ]
机构
[1] Univ Munich, Inst Physiol, D-80336 Munich, Germany
关键词
connexin; PMN; leukocyte; HUVEC; emigration; diapedesis;
D O I
10.1189/jlb.0402184
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Communication between leukocytes and endothelial cells is crucial for inflammatory reactions. Paracrine cross-talk and outside-in signaling (via adhesion molecules) have been characterized as communication pathways to date. As leukocytes and endothelial cells express connexins, we considered intercellular communication via gap junctions an intriguing additional concept. We found that gap-junctional coupling between neutrophils and endothelium occurred in a time-dependent, bidirectional manner and was facilitated by adhesion. After blockade of connexins, transmigration of nentrophils through the endothelial layer was enhanced, and the barrier function of cell monolayers was reduced during transmigration. Tumor necrosis factor alpha decreased coupling. In the presence of connexins, transmigration of neutrophils did not alter permeability. Thus, nentrophils couple to endothelium. via gap junctions, functionally modulating transmigration and leakiness. Gap-junctional coupling may he a novel way of leukocyte-endothelial communication.
引用
收藏
页码:118 / 126
页数:9
相关论文
共 31 条
[1]  
Bruzzone S, 2001, FASEB J, V15, P10
[2]   The endothelial component of cannabinoid-induced relaxation in rabbit mesenteric artery depends on gap junctional communication [J].
Chaytor, AT ;
Martin, PEM ;
Evans, WH ;
Randall, MD ;
Griffith, TM .
JOURNAL OF PHYSIOLOGY-LONDON, 1999, 520 (02) :539-550
[3]   Gap-junctional coupling measured by flow cytometry [J].
Czyz, J ;
Irmer, U ;
Schulz, G ;
Mindermann, A ;
Hülser, DF .
EXPERIMENTAL CELL RESEARCH, 2000, 255 (01) :40-46
[4]   Spatial and temporal regulation of gap junction connexin43 in vascular endothelial cells exposed to controlled disturbed flows in vitro [J].
DePaola, N ;
Davies, PF ;
Pritchard, WF ;
Florez, L ;
Harbeck, N ;
Polacek, DC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :3154-3159
[5]   Molecular mechanisms that control leukocyte extravasation: the selectins and the chemokines [J].
Ebnet, K ;
Vestweber, D .
HISTOCHEMISTRY AND CELL BIOLOGY, 1999, 112 (01) :1-23
[6]   Human T-cell lymphotropic virus type 1-transformed cells induce angiogenesis and establish functional gap junctions with endothelial cells [J].
El-Sabban, ME ;
Abou Merhi, R ;
Abi Haidar, H ;
Arnulf, B ;
Khoury, H ;
Basbous, J ;
Nijmeh, J ;
de Thé, H ;
Hermine, O ;
Bazarbachi, A .
BLOOD, 2002, 99 (09) :3383-3389
[7]   SPECIFIC PERMEABILITY AND SELECTIVE FORMATION OF GAP JUNCTION CHANNELS IN CONNEXIN-TRANSFECTED HELA-CELLS [J].
ELFGANG, C ;
ECKERT, R ;
LICHTENBERGFRATE, H ;
BUTTERWECK, A ;
TRAUB, O ;
KLEIN, RA ;
HULSER, DF ;
WILLECKE, K .
JOURNAL OF CELL BIOLOGY, 1995, 129 (03) :805-817
[8]  
GUINAN EC, 1988, AM J PATHOL, V132, P406
[9]  
He DS, 2000, CIRC RES, V86, pE104
[10]  
Hillis GS, 1997, J PATHOL, V182, P373