A Comparison of the VP1, VP2, and VP4 Regions for Molecular Typing of Human Enteroviruses

被引：49

作者：

Perera, David ^{[1
]}

Shimizu, Hiroyuki ^{[2
]}

Yoshida, Hiromu ^{[2
]}

Phan Van Tu ^{[3
]}

Ishiko, Hiroaki ^{[4
]}

McMinn, Peter C. ^{[5
]}

Cardosa, Mary J. ^{[1
]}

机构：

[1] Univ Malaysia Sarawak, Inst Hlth & Community Med, Kota Samarahan 94300, Sarawak, Malaysia

[2] Natl Inst Infect Dis, Dept Virol 2, Tokyo, Japan

[3] Pasteur Inst Ho Chi Minh City, Ho Chi Minh City, Vietnam

[4] Mitsubishi Chem Med Corp, Host Def Lab, Tokyo, Japan

[5] Univ Sydney, Cent Clin Sch, Discipline Infect Dis & Immunol, Sydney, NSW 2006, Australia

来源：

JOURNAL OF MEDICAL VIROLOGY | 2010年 / 82卷 / 04期

基金：

英国惠康基金;

关键词：

human enterovirus; RT-PCR detection; hand; foot; and mouth disease; MOUTH-DISEASE; EVOLUTION; IDENTIFICATION; EPIDEMIOLOGY; STRAINS; FOOT; HAND; PCR; PREFECTURE; HERPANGINA;

D O I：

10.1002/jmv.21652

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

The VP4, VP2, and VP1 gene regions were evaluated for their usefulness in typing human enteroviruses. Three published RT-PCR primers sets targeting separately these three gene regions were used. Initially, from a total of 86 field isolates (36 HEV-A 40 HEV-B, and 10 HEV-C) tested, 100% concordance in HEV-A was identified from all three gene regions (VP4, VP2, and VP1). However, for HEV-B and HEV-C viruses, only the VP2 and VP1 regions, and not VP4, showed 100% concordance in typing these viruses. To evaluate further the usefulness of VP4 in typing HEV-A enteroviruses, 55 Japanese and 203 published paired VP4 and VP1 nucleotide sequences were also examined. In each case, typing by VP4 was 100% in concordance with typing using VP1. Given these results, it is proposed that for HEV-A enteroviruses, all three gene regions (VP4, VP2, and VP1), would be useful for typing these viruses. These options would enhance the capability of laboratories in identifying these viruses and would greatly help in outbreaks of hand, foot, and mouth disease. J. Med. Virol. 82:649-657, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：649 / 657

页数：9

共 27 条

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