The carboxy-terminus of protein 4.1R resembles beta-tubulin

被引：2

作者：

Fant, X ^{[1
]}

Merdes, A ^{[1
]}

机构：

[1] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Inst Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

来源：

CELL BIOLOGY INTERNATIONAL | 2002年 / 26卷 / 04期

基金：

英国惠康基金;

关键词：

microtubules; NuMA; centrosome; mitosis;

D O I：

10.1006/cbir.2002.0866

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The protein 4.1R is an isoform of a larger family of 4.1 proteins. It is known as a component of the plasma membrane skeleton, but it is also found at the centrosomes in interphase and mitosis. To investigate the properties of the carboxy terminal region of protein 4.1R, we raised antibodies against a peptide representing the last 14 amino acids of 4.1R. These antibodies crossreact with an epitope in beta-tubulin and stain the microtubule network by immunofluorescence. Furthermore, sequence comparison of the carboxy terminal 4.1R peptide sequence with tubulin reveals homology with a region at the end of helix 5 in beta-tubulin, but not alpha-tubulin. A potential function of the 4.1R carboxy terminus in regulating the formation of microtubule networks is discussed. (C) 2002 Elsevier Science Ltd. All rights reserved.

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页码：371 / 377

页数：7

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