Immunomagnetic-electrochemiluminescent detection of Bacillus anthracis spores in soil matrices

被引：39

作者：

Bruno, JG ^{[1
]}

Yu, H ^{[1
]}

机构：

[1] USA,EDGEWOOD RES DEV & ENGN CTR,SYST RES LABS,ABERDEEN PROVING GROUND,MD 21010

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 09期

关键词：

D O I：

10.1128/AEM.62.9.3474-3476.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rapid (less than or equal to 1.5-h) detection of anthrax spores in soil suspensions was accomplished by an immunomagnetic electrochemiluminescence method. Strain-dependent detection limits in the range of 10(2) to 10(5) spores were achieved in buffer. The rank order of sensitivity for the assay in buffer was Sterne > Ames > Vollum 1B. Detection was up to 3 orders of magnitude less sensitive in soil suspensions, and the rank order of sensitivity was altered.

引用

页码：3474 / 3476

页数：3

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