Mutation of p53 in squamous cell cancer of the head and neck: Relationship to tumor cell proliferation

被引:23
作者
Wood, NB
Kotelnikov, V
Caldarelli, DD
Hutchinson, J
Panje, WR
Hedge, P
Leurgans, S
LaFollette, S
Taylor, SG
Preisler, HD
Coon, JS
机构
[1] RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT OTOLARYNGOL,CHICAGO,IL 60612
[2] RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT PREVENT MED,CHICAGO,IL 60612
[3] RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT MED,DIV HEMATOL ONCOL,CHICAGO,IL 60612
[4] RUSH PRESBYTERIAN ST LUKES MED CTR,RUSH CANC INST,CHICAGO,IL 60612
关键词
D O I
10.1097/00005537-199706000-00019
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Rapid proliferation of squamous cell carcinomas of the head and neck (SCCHN) during therapy may contribute to treatment failure. We have investigated the presence of p53 abnormalities in patients with SCCHN as a correlate of proliferation rate and other pathologic and clinical variables. p53 Mutation, as determined by polymerase chain reaction and single-strand conformation polymorphism analysis of microdissected frozen sections of tumor biopsies, was significantly associated with a high labeling index, as determined by in vivo infusion of IUdR and BrdU (P = 0.017), p53 Protein expression was detected by immunohistochemistry with two different antibodies, followed by quantitative image analysis. Many cases exhibited strong p53 protein expression in the absence of mutations within the conserved region of the gene, and expression was not related to proliferation. The presence of p53 mutations was related to tumor differentiation in this group of patients.
引用
收藏
页码:827 / 833
页数:7
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