Substrate activation behaviour of pyruvate decarboxylase from Pisum sativum cv Miko

被引:15
作者
Dietrich, A [1 ]
Konig, S [1 ]
机构
[1] UNIV HALLE WITTENBERG,INST BIOCHEM,FACHBEREICH BIOCHEM BIOTECHNOL,HALLE,GERMANY
关键词
substrate activation; pyruvate decarboxylase; SH-group; modification; thiamine diphosphate; Pisum sativum cv Miko;
D O I
10.1016/S0014-5793(96)01326-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The substrate activation behaviour of pyruvate decarboxylase from germinating seeds of Pisum sativum is characterised kinetically via stopped-flow measurements and discussed with respect to other species, The involvement of SH-groups in this process is demonstrated by reference experiments with chemically modified enzyme.
引用
收藏
页码:42 / 44
页数:3
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