Substrate activation behaviour of pyruvate decarboxylase from Pisum sativum cv Miko

被引：15

作者：

Dietrich, A ^{[1
]}

Konig, S ^{[1
]}

机构：

[1] UNIV HALLE WITTENBERG,INST BIOCHEM,FACHBEREICH BIOCHEM BIOTECHNOL,HALLE,GERMANY

来源：

FEBS LETTERS | 1997年 / 400卷 / 01期

关键词：

substrate activation; pyruvate decarboxylase; SH-group; modification; thiamine diphosphate; Pisum sativum cv Miko;

D O I：

10.1016/S0014-5793(96)01326-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The substrate activation behaviour of pyruvate decarboxylase from germinating seeds of Pisum sativum is characterised kinetically via stopped-flow measurements and discussed with respect to other species, The involvement of SH-groups in this process is demonstrated by reference experiments with chemically modified enzyme.

引用

页码：42 / 44

页数：3

共 12 条

[1] CATALYTIC POWER OF PYRUVATE DECARBOXYLASE - RATE-LIMITING EVENTS AND MICROSCOPIC RATE CONSTANTS FROM PRIMARY CARBON AND SECONDARY HYDROGEN ISOTOPE EFFECTS
ALVAREZ, FJ
ERMER, J
HUBNER, G
SCHELLENBERGER, A
SCHOWEN, RL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (22) : 8402 - 8409
[2] SUBSTRATE ACTIVATION OF BREWERS-YEAST PYRUVATE DECARBOXYLASE IS ABOLISHED BY MUTATION OF CYSTEINE-221 TO SERINE
BABURINA, I
GAO, YH
HU, ZX
JORDAN, F
HOHMANN, S
FUREY, W
[J]. BIOCHEMISTRY, 1994, 33 (18) : 5630 - 5635
[3] SPECTROPHOTOMETRIC STUDY OF THE REACTION OF PROTEIN SULFHYDRYL GROUPS WITH ORGANIC MERCURIALS
BOYER, PD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1954, 76 (17) : 4331 - 4337
[4] CALCULATION OF PROTEIN EXTINCTION COEFFICIENTS FROM AMINO-ACID SEQUENCE DATA
GILL, SC
VONHIPPEL, PH
[J]. ANALYTICAL BIOCHEMISTRY, 1989, 182 (02) : 319 - 326
[5] HOLZER H, 1956, BIOCHEM Z, V327, P331
[6] HUBNER G, 1988, BIOMED BIOCHIM ACTA, V47, P9
[7] MECHANISM OF SUBSTRATE ACTIVATION OF PYRUVATE DECARBOXYLASE - 1ST APPROACH
HUBNER, G
WEIDHASE, R
SCHELLENBERGER, A
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 92 (01): : 175 - 181
[8] HUBNER G, 1986, BIOCHEM INT, V13, P767
[9] KINETIC ASPECTS OF REGULATION OF PYRUVIC DECARBOXYLASE
KENWORTHY, P
DAVIES, DD
[J]. PHYTOCHEMISTRY, 1976, 15 (02) : 279 - 282
[10] PYRUVATE DECARBOXYLASE FROM ZEA-MAYS-L .2. EXAMINATION OF HYSTERETIC KINETICS
LANGSTONUNKEFER, PJ
LEE, TC
[J]. PLANT PHYSIOLOGY, 1985, 79 (02) : 436 - 440

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