Signal amplification on planar and gel-type sensor surfaces in surface plasmon resonance-based detection of prostate-specific antigen

被引:84
作者
Besselink, GAJ
Kooyman, RPH
van Os, PJHJ
Engbers, GHM
Schasfoort, RBM
机构
[1] Univ Twente, MESA Res Inst, Dept Sci & Technol, NL-7500 AE Enschede, Netherlands
[2] Univ Twente, Dept Sci & Technol, Inst Biomed Engn, NL-7500 AE Enschede, Netherlands
[3] Ssens BV, NL-7554 RS Hengelo, Netherlands
关键词
surface plasmon resonance; prostate-specific antigen; amplification; colloidal gold; latex micro spheres;
D O I
10.1016/j.ab.2004.05.009
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This article describes surface plasmon resonance (SPR)-based detection of prostate-specific antigen (PSA), comparing amplification with colloidal gold (10 nm diameter) and latex microspheres (120 nm diameter) on planar- and gel-type sensor surfaces. As matrix, 3% BSA in PBS was used. Experimental data were compared with model calculations that predict the SPR signal that results from covering of the different sensor surfaces with each of the particles used. Amplification with latex particles gave a higher signal than did that with colloidal gold. However, the limit of detection (LOD) attained by latex amplification was not as good as that obtained after gold amplification, and this was unexpected. LOD and sensitivity of the amplified PSA assays when performed with the planar-type sensor disc were equally good or better compared with those when performed with the gel-type sensor disc. Indirect evidence indicates a restricted accessibility of the gel layer on the gel-type sensor toward the colloidal gold. Application of colloidal gold led to a sensitivity increase of approximately three orders of magnitude compared with nonamplified detection. The corresponding LOD was approximately 0. 15 ng PSA/ml, which is sufficient for measuring enhanced, clinically relevant PSA levels (> 4 ng/ml). (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:165 / 173
页数:9
相关论文
共 26 条
  • [1] Development and validation of a quantitative ELISA for the measurement of PSA concentration
    Acevedo, B
    Perera, Y
    Ruiz, M
    Rojas, G
    Benítez, J
    Ayala, M
    Gavilondo, J
    [J]. CLINICA CHIMICA ACTA, 2002, 317 (1-2) : 55 - 63
  • [2] Black MH, 1999, CLIN CHEM, V45, P347
  • [3] THE RESONANT MIRROR - A NOVEL OPTICAL SENSOR FOR DIRECT SENSING OF BIOMOLECULAR INTERACTIONS .2. APPLICATIONS
    BUCKLE, PE
    DAVIES, RJ
    KINNING, T
    YEUNG, D
    EDWARDS, PR
    POLLARDKNIGHT, D
    LOWE, CR
    [J]. BIOSENSORS & BIOELECTRONICS, 1993, 8 (7-8) : 355 - 363
  • [4] Kinetic analysis of the mass transport limited interaction between the tyrosine kinase Ick SH2 domain and a phosphorylated peptide studied by a new cuvette-based surface plasmon resonance instrument
    de Mol, NJ
    Plomp, E
    Fischer, MJE
    Ruijtenbeek, R
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 279 (01) : 61 - 70
  • [5] Direct kinetic assay of interactions between small peptides and immobilized antibodies using a surface plasmon resonance biosensor
    Gomes, P
    Andreu, D
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 259 (1-2) : 217 - 230
  • [6] Haese A, 1999, ANTICANCER RES, V19, P2641
  • [7] Surface plasmon resonance sensors: review
    Homola, J
    Yee, SS
    Gauglitz, G
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 1999, 54 (1-2) : 3 - 15
  • [8] OPTICAL CONSTANTS OF NOBLE METALS
    JOHNSON, PB
    CHRISTY, RW
    [J]. PHYSICAL REVIEW B, 1972, 6 (12) : 4370 - 4379
  • [9] Rapid detection of elevated prostate-specific antigen levels in blood: Performance of various membrane strip tests compared
    Jung, K
    Zachow, J
    Lein, M
    Brux, B
    Sinha, P
    Lenk, S
    Schnorr, D
    Loening, SA
    [J]. UROLOGY, 1999, 53 (01) : 155 - 160
  • [10] Junker R, 1999, ANTICANCER RES, V19, P2625