Optimization of submerged culture conditions for the production of angiotensin converting enzyme inhibitor from Flammulina velutipes

被引：15

作者：

Kim, JM

Ra, KS

Noh, DO

Suh, HJ

机构：

[1] Korea Univ, Coll Hlth Sci, Dept Food & Nutr, Sungbuk Ku, Seoul 136703, South Korea

[2] Taegu Tech Coll, Dept Food & Nutr, Taegu 704721, South Korea

[3] Kaya Univ, Sch Human Ecol, Koryong 717800, South Korea

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2002年 / 29卷 / 05期

关键词：

Flammulina velutipes; submerged fermentation; optimization; ACE inhibitor;

D O I：

10.1038/sj.jim.7000306

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The angiotensin-converting enzyme (ACE) inhibitory effect was tested in the culture broth from submerged mycelial cultures of 20 basidiomycetes. The ACE inhibitory effect of culture broth from Flammulina velutipes strain 414 was the highest (52.8%), followed by Lentinus edodes strains 2 (44.4%) and 16 (41.3%). Nutritional requirements for the production of ACE inhibitory substance from F. velutipes were studied. Sucrose, ammonium acetate, and glutamic acid were chosen for the maximum production of ACE inhibitory substance. The optimal medium composition was (g/l): sucrose 20, ammonium acetate 5, glutamic acid 2, KH2PO4 3, MgSO4.7H(2)O 0.8, and yeast extract 0.5. Under optimal culture conditions, the ACE inhibitory effect was more than 80%.

引用

页码：292 / 295

页数：4

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