Simultaneous assessment of average fragment size and amount in minute samples of degraded DNA

被引：17

作者：

Colotte, Marthe ^{[1
,2
]}

Couallier, Vincent ^{[3
]}

Tuffet, Sophie ^{[4
]}

Bonnet, Jacques ^{[1
,2
]}

机构：

[1] Univ Bordeaux 2, INSERM, U916, Inst Bergonie, F-33076 Bordeaux, France

[2] Univ Bordeaux 2, Plant Forme Genom Fonct, F-33076 Bordeaux, France

[3] Univ Bordeaux 2, UMR 5251, Inst Math Bordeaux, F-33076 Bordeaux, France

[4] Soc IMAGENE, F-33600 Pessac, France

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 388卷 / 02期

关键词：

DNA degradation; Minute amounts; DNA size determination; DNA quantification; GMO; REAL-TIME PCR; NUCLEAR; QUANTIFICATION; TOOL;

D O I：

10.1016/j.ab.2009.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is currently no method allowing routine characterization of minute amounts of degraded DNA samples Such is those encountered in forensic science, archived tissues, ancient DNA, extracellular or stool DNA, and processed food. Here we describe and directly validate such a method based, on the one hand, oil a generalized DNA random fragmentation model and, on the other, oil two quantitative polymerase chain reaction (PCR) experiments using two different target sizes. The model also makes it possible to determine the minimum sample amount, the minimum mass average fragment size, and the maximum degradation time necessary to obtain a positive PCR. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：345 / 347

页数：3

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