Glutathione oxidation in real time by thermospray liquid chromatography-mass spectrometry

被引:27
作者
Deutsch, JC
Santhosh-Kumar, CR
Kolhouse, JF
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Med, Div Hematol, Denver, CO 80220 USA
[2] Univ Colorado, Hlth Sci Ctr, Dept Med, Div Gastroenterol, Denver, CO 80220 USA
[3] Royal Hosp, Muscat, Oman
关键词
glutathione; peptides;
D O I
10.1016/S0021-9673(99)00932-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The oxidation and reduction of glutathione and oxidized glutathione were studied in real time by liquid chromatography-mass spectrometry during exposure to hydrogen peroxide and mercaptoethanol. By mass spectrometry mixed disulfides and both reversible and irreversible oxidations of sulfur to higher states (sulfinic and sulfonic acids) were directly observed during exposure to hydrogen peroxide. The irreversible oxidation of glutathione to glutathione sulfonic acid could be detected after 30 min exposure of glutathione to 30 mM H2O2 at 20 degrees C. A peak consistent with glutathione-sulfinic acid was transiently present, suggesting this compound behaved as an oxygen consuming antioxidant. Liquid chromatography-mass spectrometry appears to be an excellent method to study oxidation and reductions of sulfur containing peptides and amino acids. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:161 / 168
页数:8
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