Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser

被引：32

作者：

Svoboda, K ^{[1
]}

Denk, W ^{[1
]}

Knox, WH ^{[1
]}

Tsuda, S ^{[1
]}

机构：

[1] AT&T BELL LABS, LUCENT TECHNOL, HOLMDEL, NJ 07733 USA

来源：

OPTICS LETTERS | 1996年 / 21卷 / 17期

关键词：

D O I：

10.1364/OL.21.001411

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A Cr:LiSrAlFl laser, pumped with a diffraction-limited laser diode and mode locked with a saturable Bragg reflector, produces 90-fs pulses at 860 nm with a cw power as high as 88 mW in two beams. It is shown that this recently developed, compact, solid-state laser can be used as an excitation source for two-photon laser scanning microscopy. Morphological and functional images of neocortical and cerebellar neurons were obtained with submicrometer three-dimensional resolution. Single dendritic spines could easily be resolved deep in scattering tissue. (C) 1996 Optical Society of America

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页码：1411 / 1413

页数：3

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