Growth of polychlorinated-biphenyl-degirading bacteria in the presence of biphenyl and chlorobiphenyls generates oxidative stress and massive accumulation of inorganic polyphosphate

被引:79
作者
Chávez, FP
Lünsdorf, H
Jerez, CA
机构
[1] Univ Chile, Dept Biol, Fac Ciencias, Lab Mol Microbiol & Biotechnol, Santiago, Chile
[2] Univ Chile, Dept Biol, Fac Ciencias, Millennium Inst Adv Studies Cell Biol & Biotechno, Santiago, Chile
[3] GBF Natl Res Ctr Biotechnol, Div Microbiol, Braunschweig, Germany
关键词
D O I
10.1128/AEM.70.5.3064-3072.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Inorganic polyphosphate (polyP) plays a significant role in increasing bacterial cell resistance to unfavorable environmental conditions and in regulating different biochemical processes. Using transmission electron microscopy of the polychlorinated biphenyl (PCB)-degrading bacterium Pseudomonas sp. strain B4 grown in defined medium with biphenyl as the sole carbon source, we observed large and abundant electron-dense granules at all stages of growth and following a shift from glucose to biphenyl or chlorobiphenyls. Using energy dispersive X-ray analysis and electron energy loss spectroscopy, with an integrated energy-filtered transmission electron microscope, we demonstrated that these granules were mainly composed of phosphate. Using sensitive enzymatic methods to quantify cellular polyP, we confirmed that this polymer accumulates in PCB-degrading bacteria when they grow in the presence of biphenyl and chlorobiphenyls. Concomitant increases in the levels of the general stress protein GroEI and reactive oxygen species were also observed in chlorobiphenyl-grown cells, indicating that these bacteria adjust their physiology with a stress response when they are confronted with compounds that serve as carbon and energy sources and at the same time are chemical stressors.
引用
收藏
页码:3064 / 3072
页数:9
相关论文
共 38 条
[1]  
AHN KH, 1990, J BIOL CHEM, V265, P11734
[2]  
Ault-Riché D, 1998, J BACTERIOL, V180, P1841
[3]   THE ELEMENTAL COMPOSITION DYNAMICS OF LARGE POLYPHOSPHATE GRANULES IN ACINETOBACTER STRAIN 210A [J].
BONTING, CFC ;
KORTSTEE, GJJ ;
BOEKESTEIN, A ;
ZEHNDER, AJB .
ARCHIVES OF MICROBIOLOGY, 1993, 159 (05) :428-434
[4]   The exopolyphosphatase gene from Sulfolobus solfataricus:: Characterization of the first gene found to be involved in polyphosphate metabolism in Archaea [J].
Cardona, ST ;
Chávez, FP ;
Jerez, CA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (10) :4812-4819
[5]   Induction of stress shock proteins DnaK and GroEL by phenoxyherbicide 2,4-D in Burkholderia sp YK-2 isolated from rice field [J].
Cho, YS ;
Park, SH ;
Kim, CK ;
Oh, KH .
CURRENT MICROBIOLOGY, 2000, 41 (01) :33-38
[6]  
CROOKE E, 1994, J BIOL CHEM, V269, P6290
[7]   Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species [J].
Crow, JP .
NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 1997, 1 (02) :145-157
[8]  
Goldberg J, 2001, MICROBIOS, V106, P177
[9]  
Gonzalez H, 1998, MICROBIOS, V93, P179
[10]  
He YY, 2002, PHOTOCHEM PHOTOBIOL, V76, P188, DOI 10.1562/0031-8655(2002)076<0188:AOCTUB>2.0.CO