Comparison of polymorphonuclear cells from healthy donors and differentiated HL-60 cells as phagocytes in an opsonophagocytic assay using antigen-coated fluorescent beads

被引：6

作者：

Guy, B ^{[1
]}

Testart, C ^{[1
]}

Gimenez, S ^{[1
]}

Sanchez, V ^{[1
]}

Lheritier, P ^{[1
]}

Rossin, D ^{[1
]}

Mignon, M ^{[1
]}

Danve, B ^{[1
]}

Trannoy, E ^{[1
]}

机构：

[1] Aventis Pasteur, Dept Res, F-69280 Marcy Letoile, France

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2000年 / 7卷 / 02期

关键词：

D O I：

10.1128/CDLI.7.2.314-317.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Polymorphonuclear cells (PMNs) from healthy donors and differentiated HL-60 cells were compared in an opsonophagocytic assay using fluorescent latex beads coated with Streptococcus pneumoniae polysaccharide conjugates, Serum-specific phagocytosis was efficiently mediated by both sources of cells, as measured by how cytometry, but the mean number of beads ingested per cell was three- to fivefold higher when PMNs were used than when HL-60 cells were used. Nevertheless, differentiated HL-60 cells could be a convenient and standardized source of cells to evaluate the functionality of specific antibodies to vaccine candidates as a coating on fluorescent beads.

引用

页码：314 / 317

页数：4

共 6 条

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