The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

被引：39

作者：

Sunna, A

Gibbs, MD

Bergquist, PL ^{[1
]}

机构：

[1] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia

[2] Univ Auckland, Sch Med, Dept Mol Med, Auckland, New Zealand

来源：

BIOCHEMICAL JOURNAL | 2000年 / 346卷 / pt 3期

关键词：

cellulose binding domains; glycosyl hydrolases; multidomains; TSD;

D O I：

10.1042/0264-6021:3460583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that the N-terminal 'thermostabilizing domain ' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

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页码：583 / 586

页数：4

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