Reliability of HSP70 (HSPA) expression as a prognostic marker in glioma

被引：37

作者：

Beaman, Glenda Maria ^{[1
]}

Dennison, Sarah R. ^{[2
]}

Chatfield, Lee K. ^{[3
]}

Phoenix, David A. ^{[4
]}

机构：

[1] Univ Cent Lancashire, Sch Forens & Investigat Sci, Preston PR1 2HE, Lancs, England

[2] Univ Cent Lancashire, Sch Pharm & Biomed Sci, Preston PR1 2HE, Lancs, England

[3] Univ Cent Lancashire Cyprus, CY-7080 Larnax, Cyprus

[4] London South Bank Univ, London SE1 0AA, England

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2014年 / 393卷 / 1-2期

关键词：

Glioma; Biomarker; Gene expression; Heat shock protein 70 (HSP70/HSPA); HEAT-SHOCK PROTEINS; NEGATIVE BREAST-CANCER; HEAT-SHOCK-PROTEIN-70; BRAIN; CELLS; SURVIVAL; FAMILY; INHIBITION; CHAPERONES; APOPTOSIS;

D O I：

10.1007/s11010-014-2074-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Production of heat shock protein 70 (HSP70/HSPA) is induced by a wide range of cellular stress conditions, such as cancer and hypoxia, with production also being linked to tumourigenesis. HSPA mRNA transcripts and proteins were examined in three human glioma cell lines, representing astrocytoma, oligodendroglioma and glioblastoma, plus 18 clinical brain tissue samples. GAPDH was used as a control gene throughout these studies and exhibited a consistent level of expression in a normal astrocyte cell line, tumourous cell lines and tissue samples. In contrast, the average HSPA mRNA copy numbers detected in glioblastoma tissue were between 1.8- and 8.8-fold higher than in lower grade glioma and control tissue, respectively, which is suggestive of a grade-related transcription profile. Similar patterns of grade-related expression were also observed in glioma cell lines. This study indicates for the first time that HSPA expression in glioma cells may possibly be grade related, and hence could have potential as a prognostic marker.

引用

页码：301 / 307

页数：7

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