Chemoenzymatic ubiquitination of artificial substrates

被引：17

作者：

Burchak, Olga N.

Jaquinod, Michel

Cottin, Claire

Mugherli, Laurent

Iwai, Kazuhiro

Chatelain, Francois

Balakirev, Maxim Y.

机构：

[1] CEA, Dept Response & Dynam Cellulaires, F-38054 Grenoble, France

[2] Osaka City Univ, Grad Sch Med, Dept Mol & Cell Biol, Abeno Ku, Osaka 5458585, Japan

来源：

CHEMBIOCHEM | 2006年 / 7卷 / 11期

关键词：

enzymes; ligation; microarrays; thioesters; ubiquitin;

D O I：

10.1002/cbic.200600283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Probing an alternative route. Ubiquitin and Ub-like proteins (Ubl) are attached to cellular proteins through a conserved readily transferred onto cysteine-containing artificial substrates. This finding provides new insight into the chemistry conjugation pathway. We demonstrate of Ub (Ubl) conjugation and can be here that Ub and Ubl thioesters, the key used for creating different protein intermediates of this pathway, can be probes and microarrays.

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页码：1667 / +

页数：4

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