Genome sequence survey identifies unique sequences and key virulence genes with unusual rates of amino acid substitution in bovine Staphylococcus aureus

被引:45
作者
Herron, LL
Chakravarty, R
Dwan, C
Fitzgerald, JR
Musser, JM
Retzel, E
Kapur, V
机构
[1] Univ Minnesota, Dept Vet Pathobiol, St Paul, MN 55108 USA
[2] Univ Minnesota, Biomed Genom Ctr, St Paul, MN 55108 USA
[3] Univ Minnesota, Computat Biol Ctr, Minneapolis, MN 55455 USA
[4] NIAID, Rocky Mt Labs, Lab Human Bacterial Pathogenesis, NIH, Hamilton, MT 59840 USA
关键词
D O I
10.1128/IAI.70.7.3978-3981.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Staphylococcus aureus is a major cause of mastitis in bovine and other ruminant species. We here present the results of a comparative genomic analysis between a bovine mastitis-associated clone, RF122, and the recently sequenced human-associated clones, Mu50 and N315, of Staphylococcus aureus. A shotgun sequence survey of similar to10% of the RF122 genome identified numerous unique sequences and those with elevated rates of nonsynonymous substitution. Taken together, these analyses show that there are notable differences in the genomes of bovine mastitis-associated and human clones of S. aureus and provide a framework for the identification of specific factors associated with host specificity in this major human and animal pathogen.
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收藏
页码:3978 / 3981
页数:4
相关论文
共 15 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   Bap, a Staphylococcus aureus surface protein involved in biofilm formation [J].
Cucarella, C ;
Solano, C ;
Valle, J ;
Amorena, B ;
Lasa, I ;
Penadés, JR .
JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2888-2896
[3]   Molecular population and virulence factor analysis of Staphylococcus aureus from bovine intramammary infection [J].
Fitzgerald, JR ;
Hartigan, PJ ;
Meaney, WJ ;
Smyth, CJ .
JOURNAL OF APPLIED MICROBIOLOGY, 2000, 88 (06) :1028-1037
[4]   Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens [J].
Fitzgerald, JR ;
Monday, SR ;
Foster, TJ ;
Bohach, GA ;
Hartigan, PJ ;
Meaney, WJ ;
Smyth, CJ .
JOURNAL OF BACTERIOLOGY, 2001, 183 (01) :63-70
[5]   MOLECULAR POPULATION GENETIC-ANALYSIS OF STAPHYLOCOCCUS-AUREUS RECOVERED FROM COWS [J].
KAPUR, V ;
SISCHO, WM ;
GREER, RS ;
WHITTAM, TS ;
MUSSER, JM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) :376-380
[6]   Whole genome sequencing of meticillin-resistant Staphylococcus aureus [J].
Kuroda, M ;
Ohta, T ;
Uchiyama, I ;
Baba, T ;
Yuzawa, H ;
Kobayashi, I ;
Cui, LZ ;
Oguchi, A ;
Aoki, K ;
Nagai, Y ;
Lian, JQ ;
Ito, T ;
Kanamori, M ;
Matsumaru, H ;
Maruyama, A ;
Murakami, H ;
Hosoyama, A ;
Mizutani-Ui, Y ;
Takahashi, NK ;
Sawano, T ;
Inoue, R ;
Kaito, C ;
Sekimizu, K ;
Hirakawa, H ;
Kuhara, S ;
Goto, S ;
Yabuzaki, J ;
Kanehisa, M ;
Yamashita, A ;
Oshima, K ;
Furuya, K ;
Yoshino, C ;
Shiba, T ;
Hattori, M ;
Ogasawara, N ;
Hayashi, H ;
Hiramatsu, K .
LANCET, 2001, 357 (9264) :1225-1240
[7]   Complete genomic sequence of Pasteurella multocida, Pm70 [J].
May, BJ ;
Zhang, Q ;
Li, LL ;
Paustian, ML ;
Whittam, TS ;
Kapur, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :3460-3465
[8]   Identification, cloning, and initial characterization of rot, a locus encoding a regulator of virulence factor expression in Staphylococcus aureus [J].
McNamara, PJ ;
Milligan-Monroe, KC ;
Khalili, S ;
Proctor, RA .
JOURNAL OF BACTERIOLOGY, 2000, 182 (11) :3197-3203
[9]   Cell wall-affecting antibiotics induce expression of a novel gene, drp35, in Staphylococcus aureus [J].
Murakami, H ;
Matsumaru, H ;
Kanamori, M ;
Hayashi, H ;
Ohta, T .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 264 (02) :348-351
[10]  
Musser J. M., 1990, MOL BIOL STAPHYLOCOC, P59