Heat-induced antigen reteieval: What are we retrieving?

被引：27

作者：

Boenisch, Thomas ^{[1
]}

机构：

[1] DakoCytomat RD, Carpinteria, CA 93013 USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2006年 / 54卷 / 09期

关键词：

antigen retrieval; epitope unmasking; immunohisotochemistry;

D O I：

10.1369/jhc.6P6945.2006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Of all molecular aspects lost and recovered during formalin fixation and antigen retrieval, respectively, electrostatic charges have probably received the least attention. This review will focus on our work during the past 7 years. It strongly supports the tenet that electrostatic charges, i.e., net negative on antigens and net positive on antibodies, play an important part in immune reactions and therefore should be given greater attention when focusing on quality control in immunohistochemistry.

引用

页码：961 / 964

页数：4

共 8 条

[1]

[Anonymous], 1984, MOL IMMUNOLOGY TXB

[2] Effect of heat-induced antigen retrieval following inconsistent formalin fixation [J].