Photophysical properties of an on/off fluorescent pH indicator excitable with visible light based on a borondipyrromethene-linked phenol

被引：68

作者：

Qin, Wenwu ^{[1
]}

Baruah, Mukulesh ^{[1
]}

De Borggraeve, Wim M. ^{[1
]}

Boens, Noel ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Chem, B-3001 Heverlee, Belgium

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2006年 / 183卷 / 1-2期

关键词：

fluorescent probe; pH sensor; acidity constant; borondipyrromethene; spectroscopy;

D O I：

10.1016/j.jphotochem.2006.03.015

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A borondipyrromethene-derived pH indicator (available as methyl ester (1) and sodium salt (2)) for the near-neutral pH range with ultra bright fluorescence in the red spectral region has been synthesized by linking o-chlorophenol to the 3-position of a difluoroboradiazaindacene derivative. Absorption and steady-state and time-resolved fluorescence measurements have been used to study the photophysical properties of the BODIPY-based pH probe. The fluorescence lifetime (3.8 +/- 0.2 ns) and the very high (nearly 1.0) fluorescence quantum yield of dye 1 are not dependent on the solvent. In aqueous solution, the water-soluble compound 2 undergoes a reversible protonation-deprotonation reaction in the near-neutral pH range with a pK(a) of 7.60, which is practically insensitive to low ionic strength. Fluorimetric titrations as a function of pH produce fluorescence emission enhancements at lower pH. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：190 / 197

页数：8

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