A new HPLC method for serum neopterin measurement and relationships with plasma thiols levels in healthy subjects

被引:34
作者
Carru, C
Zinellu, A
Sotgia, S
Serra, R
Usai, MF
Pintus, GF
Pes, GM
Deiana, L
机构
[1] Univ Sassari, Chair Clin Biochem, I-07100 Sassari, Italy
[2] Lab Clin Anal Meridiana SRL, Sassari, Italy
[3] Univ Sassari, Dept Biomed Sci, I-07100 Sassari, Italy
关键词
high-performance liquid chromatography; neopterin; thiols; homocysteine; cysteine; cardiovascular risk;
D O I
10.1002/bmc.325
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Neopterin, a pyrazinopyrimidine compound, serves as a marker of cellular immune system activation, and it can be used as a prognostic predictor for certain types of diseases. We propose a new simple HPLC method to measure serum neopterin with highly sensitive fluorimetric detection. After TCA serum protein precipitation, the supernatant was diluted five times, injected into a C-18 reversed-phase column and eluted at a flow rate of 1.5 mL/min by an isocratic water-acetonitrile (99:1) mobile phase. The natural fluorescence of the molecule was detected at excitation wavelength 353 nm and emission 438 nm. In these conditions the neopterin retention time was about 4 min. Our proposed method was compared with a validated chromatographic separation, and the obtained data of the serum neopterin from 35 healthy volunteers were analysed by Passing-Bablok regression and Bland-Altman test. Neopterin measurement in healthy subjects was also employed to investigate on its potential relationships with plasma thiols levels. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:360 / 366
页数:7
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