Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells

被引：144

作者：

Miharada, Kenichi

Hiroyama, Takashi

Sudo, Kazuhiro

Nagasawa, Toshiro

Nakamura, Yukio

机构：

[1] RIKEN, BioResource Ctr, Cell Engn Div, Tsukuba, Ibaraki 3050074, Japan

[2] Univ Tsukuba, Inst Clin Med, Div Hematol, Tsukuba, Ibaraki 3058577, Japan

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 10期

关键词：

D O I：

10.1038/nbt1245

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Erythroblast enucleation is thought to be largely dependent on signals mediated by other cells, such as macrophages. In an attempt to improve the in vitro production of red blood cells (RBCs) from immature hematopoietic progenitor cells, we have developed a method to produce enucleated RBCs efficiently in the absence of feeder cells. Our method may represent an efficient way to produce transfusable RBCs on a large scale from hematopoietic progenitors.

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页码：1255 / 1256

页数：2

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