Identification of the glial cell types containing carnosine-related peptides in the rat brain

被引:20
作者
DeMarchis, S
Melcangi, RC
Modena, C
Cavaretta, I
Peretto, P
Agresti, C
Fasolo, A
机构
[1] UNIV TURIN,DEPT HUMAN & ANIM BIOL,I-10123 TURIN,ITALY
[2] UNIV MILAN,DEPT ENDOCRINOL,MILAN,ITALY
[3] UNIV TURIN,DEPT VET MORPHOPHYSIOL,TURIN,ITALY
[4] IST SUPER SANITA,LAB PATHOPHYSIOL,I-00161 ROME,ITALY
关键词
carnosine; central nervous system; rat; oligodendrocytes; astrocytes; cell culture; immunocytochemistry;
D O I
10.1016/S0304-3940(97)00800-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The cellular localization of carnosine-like immunoreactivity was investigated in the adult rat forebrain and in glial cell cultures obtained from newborn rat brain. Using double staining methods, we showed that in vivo carnosine-like immunoreactivity was occurring in a large number of both glial fibrillary acidic protein (GFAP)-positive astrocytes and 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP)-positive oligodendrocytes. In vitro, the carnasine-immunoreactive staining was restricted to a subpopulation of completely differentiated oligodendrocytes, whereas no reaction was detected in immature oligodendrocytes and in astrocytes. These observations could have profound physiopathological implications considering the role suggested for carnosine and related peptides as endogenous antioxidants, free radical scavengers and anti-glycating agents of the central nervous system (CNS). (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:37 / 40
页数:4
相关论文
共 17 条
[1]  
[Anonymous], [No title captured]
[2]  
BAUER K, 1982, J BIOL CHEM, V257, P3593
[3]   CELLULAR-LOCALIZATION OF CARNOSINE-LIKE AND ANSERINE-LIKE IMMUNOREACTIVITIES IN RODENT AND AVIAN CENTRAL-NERVOUS-SYSTEM [J].
BIFFO, S ;
GRILLO, M ;
MARGOLIS, FL .
NEUROSCIENCE, 1990, 35 (03) :637-651
[4]  
DUBOIS C, 1990, J BIOL CHEM, V265, P2797
[5]  
HARDY R, 1991, DEVELOPMENT, V111, P1061
[6]   Carnosine-synthesis in cultures of rat glial cells is restricted to oligodendrocytes and carnosine uptake to astrocytes [J].
Hoffmann, AM ;
Bakardjiev, A ;
Bauer, K .
NEUROSCIENCE LETTERS, 1996, 215 (01) :29-32
[7]   DIFFERENTIATION OF CEREBELLAR BIPOTENTIAL GLIAL PRECURSORS INTO OLIGODENDROCYTES IN PRIMARY CULTURE - DEVELOPMENTAL PROFILE OF SURFACE-ANTIGENS AND MITOTIC-ACTIVITY [J].
LEVI, G ;
ALOISI, F ;
WILKIN, GP .
JOURNAL OF NEUROSCIENCE RESEARCH, 1987, 18 (03) :407-417
[8]   LONG-DISTANCE NEURONAL MIGRATION IN THE ADULT MAMMALIAN BRAIN [J].
LOIS, C ;
ALVAREZBUYLLA, A .
SCIENCE, 1994, 264 (5162) :1145-1148
[9]   CARNOSINE IN PRIMARY OLFACTORY PATHWAY [J].
MARGOLIS, FL .
SCIENCE, 1974, 184 (4139) :909-911
[10]   PREPARATION OF SEPARATE ASTROGLIAL AND OLIGODENDROGLIAL CELL-CULTURES FROM RAT CEREBRAL TISSUE [J].
MCCARTHY, KD ;
DEVELLIS, J .
JOURNAL OF CELL BIOLOGY, 1980, 85 (03) :890-902