Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants

被引：50

作者：

Jordá, L ^{[1
]}

Conejero, V ^{[1
]}

Vera, P ^{[1
]}

机构：

[1] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain

来源：

PLANT PHYSIOLOGY | 2000年 / 122卷 / 01期

关键词：

D O I：

10.1104/pp.122.1.67

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersicon esculentum) plants. The two new members, P69E and P69F, are organized in a cluster and arranged in a tandem form. mRNA expression analysis and studies of transgenic Arabidopsis plants transformed with promoter-P-glucuronidase fusions for each of these two genes revealed that they are differentially regulated, with each showing a highly specific mRNA expression pattern. P69E mRNA is expressed only in roots, while P69F mRNA is expressed only in hydathodes. A comparison of all the P69 amino acid sequences, gene structure, expression profiles, and clustered organization suggests a working model for P69 gene family evolution.

引用

页码：67 / 73

页数：7

共 25 条

[1] MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES [J].

BARR, PJ .

CELL, 1991, 66 (01) :1-3

[2]

BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194

[3]

COOK AA, 1952, PHYTOPATHOLOGY, V42, P162

[4]

DALE JE, 1988, ANNU REV PLANT PHYS, V39, P267, DOI 10.1146/annurev.pp.39.060188.001411

[5] MOLECULAR COMMUNICATION IN INTERACTIONS BETWEEN PLANTS AND MICROBIAL PATHOGENS [J].

DIXON, RA ;

LAMB, CJ .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1990, 41 :339-367

[6] LOCALIZATION, CONJUGATION, AND FUNCTION OF SALICYLIC-ACID IN TOBACCO DURING THE HYPERSENSITIVE REACTION TO TOBACCO MOSAIC-VIRUS [J].

ENYEDI, AJ ;

YALPANI, N ;

SILVERMAN, P ;

RASKIN, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2480-2484

[7]

Essau K, 1965, PLANT ANATOMY

[8]

Jefferson RA., 1987, PLANT MOL BIOL REP, V5, P387, DOI DOI 10.1007/BF02667740

[9] Genomic cluster containing four differentially regulated subtilisin-like processing protease genes is in tomato plants [J].

Jordá, L ;

Coego, A ;

Conejero, V ;

Vera, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) :2360-2365

[10]

KUNKEL TA, 1987, METHOD ENZYMOL, V154, P367

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