Luminescence of functionalized carbon nanotubes as a tool to monitor bundle formation and dissociation in water: The effect of plasmid-DNA complexation

被引:41
作者
Lacerda, Lara
Pastorin, Giorgia
Wu, Wei
Prato, Maurizio
Bianco, Alberto
Kostarelos, Kostas
机构
[1] Univ London, Sch Pharm, Ctr Drug Delivery Res, London WC1N 1AX, England
[2] CNRS, Inst Biol Mol & Cellulaire, UPR9021, F-67084 Strasbourg, France
[3] Univ Trieste, Dipartimento Sci Farmaceut, I-34127 Trieste, Italy
关键词
D O I
10.1002/adfm.200500569
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Functionalized carbon nanotubes (f-CNTs) are explored as novel nanomaterials for biomedical applications. UV-vis luminescence of aqueous dispersions of CNT-NH3+ and CNT-NH-Ac (NH-Ac: acetamido) is observed using standard laboratory spectrophotometric instrumentation, and the measured fluorescence intensity is correlated with the aggregation state of the f-CNTs: a high intensity indicates improved f-CNT individualization and dispersion, while a decrease in fluorescence intensity indicates a higher degree of nanotube aggregation and bundling as a result of varying the sodium dodecyl sulfate (SDS) concentrations and pH in the aqueous phase. Moreover, utilization of this relationship between fluorescence intensity and the state of f-CNT aggregation is carried out to elucidate the interactions between f-CNTs and gene-encoding plasmid DNA (pDNA). pDNA is shown to interact with CNT-NH3+ primarily through electrostatic interactions that lead concomitantly to a higher degree of f-CNT bundling. The CNT-NH3+/pDNA interactions are successfully competed by SDS/f-CNT surface interactions, resulting in the displacement of pDNA. These studies provide exemplification of the use of fluorescence spectrophotometry to accurately describe the aggregation state of water'-soluble f-CNTs. Characterization of the complexes between pDNA and f-CNTs elucidates the opportunities and limitations of such supramolecular systems as potential vectors for gene transfer.
引用
收藏
页码:1839 / 1846
页数:8
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