Solution NMR spectroscopy beyond 25 kDa

被引：144

作者：

Kay, LE

Gardner, KH

机构：

[1] UNIV TORONTO, DEPT BIOCHEM, TORONTO, ON M5S 1A8, CANADA

[2] UNIV TORONTO, DEPT CHEM, TORONTO, ON M5S 1A8, CANADA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1997年 / 7卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(97)80084-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Improvements in NMR instrumentation, higher magnetic field strengths, novel NMR experiments and new deuterium-labeling strategies have significantly increased the scope of structural problems that can now be addressed by solution NMR methods. To date, a number of structures of proteins of 30 kDa have been solved using multidimensional N-15,C-13,H-2 NMR techniques, and this molecular weight limit will probably be surpassed in the near future. (C) Current Biology Ltd ISSN 0959-440X

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页码：722 / 731

页数：10

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