Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp strain AMB-1

被引：163

作者：

Matsunaga, Tadashi ^{[1
]}

Okamura, Yoshiko ^{[1
]}

Fukuda, Yorikane ^{[1
]}

Wahyudi, Aris Tri ^{[1
]}

Murase, Yaeko ^{[1
]}

Takeyama, Haruko ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo, Japan

来源：

DNA RESEARCH | 2005年 / 12卷 / 03期

关键词：

magnetotactic bacteria; biomineralization; magnetosome;

D O I：

10.1093/dnares/dsi002

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Magnetospirillum sp. strain AMB-1 is a Gram-negative alpha-proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in a chain. The potential of this nano-sized material is growing and will be applicable to broad research areas. It has been expected that genome analysis would elucidate the mechanism of magnetosome formation by magnetic bacteria. Here we describe the genome of Magnetospirillum sp. AMB-1 wild type, which consists of a single circular chromosome of 4 967 148 bp. For identification of genes required for magnetosome formation, transposon mutagenesis and determination of magnetosome membrane proteins were performed. Analysis of a non-magnetic transposon mutant library focused on three unknown genes from 2752 unknown genes and three genes from 205 signal transduction genes. Partial proteome analysis of the magnetosome membrane revealed that the membrane contains numerous oxidation/reduction proteins and a signal response regulator that may function in magnetotaxis. Thus, oxidation/reduction proteins and elaborate multidomain signaling proteins were analyzed. This comprehensive genome analysis will enable resolution of the mechanisms of magnetosome formation and provide a template to determine how magnetic bacteria maintain a species-specific, nanosized, magnetic single domain and paramagnetic morphology.

引用

页码：157 / 166

页数：10

共 36 条

[1] Ecological role of energy taxis in microorganisms [J].

Alexandre, G ;

Greer-Phillips, S ;

Zhulin, IB .

FEMS MICROBIOLOGY REVIEWS, 2004, 28 (01) :113-126

[2] A novel protein tightly bound to bacterial magnetic particles in Magnetospirillum magneticum strain AMB-1 [J].

Arakaki, A ;

Webb, J ;

Matsunaga, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (10) :8745-8750

[3] WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].

FLEISCHMANN, RD ;

ADAMS, MD ;

WHITE, O ;

CLAYTON, RA ;

KIRKNESS, EF ;

KERLAVAGE, AR ;

BULT, CJ ;

TOMB, JF ;

DOUGHERTY, BA ;

MERRICK, JM ;

MCKENNEY, K ;

SUTTON, G ;

FITZHUGH, W ;

FIELDS, C ;

GOCAYNE, JD ;

SCOTT, J ;

SHIRLEY, R ;

LIU, LI ;

GLODEK, A ;

KELLEY, JM ;

WEIDMAN, JF ;

PHILLIPS, CA ;

SPRIGGS, T ;

HEDBLOM, E ;

COTTON, MD ;

UTTERBACK, TR ;

HANNA, MC ;

NGUYEN, DT ;

SAUDEK, DM ;

BRANDON, RC ;

FINE, LD ;

FRITCHMAN, JL ;

FUHRMANN, JL ;

GEOGHAGEN, NSM ;

GNEHM, CL ;

MCDONALD, LA ;

SMALL, KV ;

FRASER, CM ;

SMITH, HO ;

VENTER, JC .

SCIENCE, 1995, 269 (5223) :496-512

[4] Magneto-aerotaxis in marine coccoid bacteria [J].

Frankel, RB ;

Bazylinski, DA ;

Johnson, MS ;

Taylor, BL .

BIOPHYSICAL JOURNAL, 1997, 73 (02) :994-1000

[5] MAGNETITE IN FRESHWATER MAGNETOTACTIC BACTERIA [J].

FRANKEL, RB ;

BLAKEMORE, RP ;

WOLFE, RS .

SCIENCE, 1979, 203 (4387) :1355-1356

[6]

Galperin MY, 2001, FEMS MICROBIOL LETT, V203, P11, DOI 10.1111/j.1574-6968.2001.tb10814.x

[7] Biochemical and proteomic analysis of the magnetosome membrane in Magnetospitillum gryphiswaldense [J].

Grünberg, K ;

Müller, EC ;

Otto, A ;

Reszka, R ;

Linder, D ;

Kube, M ;

Reinhardt, R ;

Schüler, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (02) :1040-1050

[8] A large gene cluster encoding several magnetosome proteins is conserved in different species of magnetotactic bacteria [J].

Grünberg, K ;

Wawer, C ;

Tebo, BM ;

Schüler, D .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (10) :4573-4582

[9] CONTROLLED BIOSYNTHESIS OF GREIGITE (FE3S4) IN MAGNETOTACTIC BACTERIA [J].

HEYWOOD, BR ;

BAZYLINSKI, DA ;

GARRATTREED, A ;

MANN, S ;

FRANKEL, RB .

NATURWISSENSCHAFTEN, 1990, 77 (11) :536-538

[10] Magnetosome vesicles are present before magnetite formation, and MamA is required for their activation [J].

Komeili, A ;

Vali, H ;

Beveridge, TJ ;

Newman, DK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (11) :3839-3844

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