Cloning of a cation efflux pump gene associated with chlorhexidine resistance in Klebsiella pneumoniae

被引：59

作者：

Fang, CT

Chen, HC

Chuang, YP

Chang, SC

Wang, JT

机构：

[1] Natl Taiwan Univ, Coll Med, Dept Microbiol, Taipei 100, Taiwan

[2] Natl Taiwan Univ Hosp, Dept Internal, Taipei, Taiwan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 06期

关键词：

D O I：

10.1128/AAC.46.6.2024-2028.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression libraries of a chlorhexidine-resistant Klebsiella pneumoniae strain were constructed and transformed into Escherichia coli XLOLR. Twenty chlorhexidine-resistant transformants were obtained after selection. All clones contained a novel 903-nucleotide locus. Its sequences were compatible with a cation efflux pump, and the locus was thus designated as cepA. Retransformation using cepA-containing plasmids conferred chlorhexidine resistance to both XLOLR and a chlorhexidine-sensitive K. pneumoniae strain. Therefore, CepA is associated with chlorhexidine resistance and may act as a cation efflux pump.

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页码：2024 / 2028

页数：5

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