Multistep microreactions with proteins using electrocapture technology

被引：24

作者：

Astorga-Wells, J ^{[1
]}

Bergman, T ^{[1
]}

Jörnvall, H ^{[1
]}

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, SE-17177 Stockholm, Sweden

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 09期

关键词：

D O I：

10.1021/ac0354342

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method to perform multistep reactions by means of electroimmobilization of a target molecule in a microflow stream is presented. A target protein is captured by the opposing effects between the hydrodynamic and electric forces, after which another medium is injected into the system. The second medium carries enzymes or other reagents, which are brought into contact with the target protein and react. The immobilization is reversed by disconnecting the electric field, upon which products are collected at the outlet of the device for analysis. On-line reduction, alkylation, and trypsin digestion of proteins is demonstrated and was monitored by MALDI mass spectrometry.

引用

页码：2425 / 2429

页数：5

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