Biphasic hierarchical extracellular matrix scaffold for osteochondral defect regeneration

被引：47

作者：

Lin, X. ^{[1
]}

Chen, J. ^{[1
]}

Qiu, P. ^{[1
]}

Zhang, Q. ^{[1
]}

Wang, S. ^{[1
]}

Su, M. ^{[1
]}

Chen, Y. ^{[1
]}

Jin, K. ^{[2
]}

Qin, A. ^{[3
]}

Fan, S. ^{[1
]}

Chen, P. ^{[1
]}

Zhao, X. ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Orthopaed Surg, Sir Run Run Shaw Hosp, Coll Med, Hangzhou, Zhejiang, Peoples R China

[2] Wenzhou Med Univ, Dept Pathophysiol, Wenzhou, Peoples R China

[3] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Orthoped, Shanghai Key Lab Orthoped Implant,Sch Med, Shanghai, Peoples R China

来源：

OSTEOARTHRITIS AND CARTILAGE | 2018年 / 26卷 / 03期

关键词：

Extracellular matrix; Osteoarthritis; Cartilage; Subchondral bone; Osteochondral defect; ARTICULAR-CARTILAGE; SUBCHONDRAL BONE; OSTEOARTHRITIS; ANGIOGENESIS; DEGENERATION; COLLAGEN; REPAIR; DECELLULARIZATION; DIFFERENTIATION; CONSTRUCTS;

D O I：

10.1016/j.joca.2017.12.001

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: To investigate the effect of decellularized osteochondral extracellular matrix (ECM) scaffold for osteochondral defect regeneration. Design: We compared the histological features and microstructure of degenerated cartilage to normal articular cartilage. We also generated and evaluated osteochondral ECM scaffolds through decellularization technology. Then scaffolds were implanted to osteochondral defect in rabbit model. After 12 weeks surgery, regeneration tissues were analyzed by histology, immunohistochemistry evaluation. And possible mechanisms of angiogenesis and cell migration were explored. Results: We demonstrated decreased cell numbers, formation of fibrous cartilage, lost microstructure and worse permeability in degenerated cartilage compared to normal cartilage. We also generated an osteochondral ECM scaffold with a hierarchical structure that exhibited low immunogenicity, high bioactivity, and well biocompatibility. We found that the ECM scaffold promoted tissue regeneration in osteochondral defects, which was dependent on the scaffold constituents and stratified threedimensional microstructure as well as on its ability to inhibit angiogenesis and stimulate cell migration. Conclusions: Our findings demonstrated that the biphasic hierarchical ECM scaffold represents a novel and effective biomaterial that can be used in the treatment of osteochondral defect. (c) 2017 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：433 / 444

页数：12

共 45 条

[1]

ECM stiffness primes the TGFβ pathway to promote chondrocyte differentiation [J].