Role of Escherichia coli DNA polymerase IV in in vivo replication fidelity

被引：57

作者：

Kuban, W

Jonczyk, P

Gawel, D

Malanowska, K

Schaaper, RM

Fijalkowska, WJ

机构：

[1] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

[2] NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 14期

关键词：

D O I：

10.1128/JB.186.14.4802-4807.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have investigated whether DNA polymerase TV (Pol IV; the dinB gene product) contributes to the error rate of chromosomal DNA replication in Escherichia coli. We compared mutation frequencies in mismatch repair-defective strains that were either dinB positive or dinB deficient, using a series of mutational markers, including lac targets in both orientations on the chromosome. Virtually no contribution of Pol IV to the chromosomal mutation rate was observed. On the other hand, a significant effect of dinB was observed for reversion of a lac allele when the lac gene resided on an F'(pro-lac) episome.

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页码：4802 / 4807

页数：6

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